hands-on-pichia: Tradition of the course

Pichia-technology achievement

The yeast Pichia pastoris (new name Komagataella phaffii) puts functional science into people’s everyday lives. Popular media proclaim the benefits of leather or meat that is no longer obtainable directly from animals but can be produced biosynthetically using this yeast species. Even luminescence effects in toys are caused by a substance produced by Pichia.

 

Most importantly, several multi-domain proteins are already being made using Pichia instead of mammalian cell cultures, and Pichia’s use to produce industrial and therapeutic enzymes is continuously increasing. Proteins such as antibodies, enzymes and peptide hormones and others require complex folding and, since they contain disulphide bonds, they are difficult to produce as soluble proteins in E. coli. In addition, novel Pichia-systems synthesize recombinant proteins containing human-like, homogenous, and therefore controllable, patterns of glycosylation. All these attributes, together with the power of highly productive bioprocesses, confirms the Pichia system’s importance for biomanufacturing of the future.

Why attend a hands-on-pichia course?

When the economic or ecological sustainability of a production process using natural animal or plant materials is unacceptable, alternative, appropriate and practicable bioprocesses can be developed using engineered Pichia. Since 2012, advances in Pichia-technology have been disseminated by dedicated experts from Austria, USA and Switzerland in a unique course that expands practical skills of its participants beyond the application of purchasable kits and published standard protocols. This course covers the basics of Pichia expression technologies and aligns them with design aspects of high-performance bioprocesses and advanced strain-design, including recent applications of CRISPR/Cas9 technologies in Pichia.

Contributing Specialists 2018

Iskandar Dib (VTU Technology GmbH, Graz, Austria)

Katharina Ebner (bisy e.U., Hofstätten/Raab, Austria)

Jasmin Fischer (bisy e.U., Hofstätten/Raab, Austria)

Anton Glieder (TU Graz, Austria)

Michaela Gerstmann (TU Graz, Austria)

Anna Hatzl (TU Graz, Austria)

Karin Kovar (daspool, Wädenswil, Switzerland)

Knut Madden (BioGrammatics Inc., Carlsbad, California, U.S.A.)

Harald Pichler (TU Graz, Austria)

Hana Raschmanova (UCT Prague, Czech Republic)

Christian Schmid (TU Graz, Austria)

Marcel Straumann (daspool, Wädenswil, Switzerland)

Gerhard Thallinger (TU Graz, Austria)

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Course Specialists 2018

Details

Course Content

In this hands-on-pichia course 2018, experts will explain and demonstrate the efficient use of the Pichia pastoris (Komagataella phaffii) expression and production system.

We will explore underlying concepts and provide insights into current laboratory practices, step-by-step protocols and novel innovative tools. In particular, a number of Pichia pastoris special features will be addressed to demonstrate how its full molecular capacity can be exploited when employed for production processes at different levels of scale. During hands-on laboratory sessions and small-group workshops, the participants will clarify and discuss suitable, tailor-made solutions for selected case studies.

Participants will be provided with supporting materials to guide them through the course.

Based on practical experience gained during this course, participants will acquire structured guidance on how to achieve optimum production performance using Pichia.

hands-on-pichia course 2018: key expertise for success

Key course topics cover all critical steps from a product idea to its production:

  • design of expression strategies
  • state-of-the-art laboratory procedures
  • crosstalk between molecular and bioprocess design

hands-on-pichia course is suitable for:

Practitioners and researchers who want to expand their level of expertise as well as practical skills beyond the use of purchasable Pichia expression kits and standard protocols. On completion of this course, participants should be able to utilise the inherent potentials of the Pichia pastoris (Komagataella phaffii) expression and production system.

Host and Main Organiser

In this hands-on-pichia course 2018, experts will explain and demonstrate the efficient use of the Pichia pastoris (Komagataella phaffii) expression and production system.

We will explore underlying concepts and provide insights into current laboratory practices, step-by-step protocols and novel innovative tools. In particular, a number of Pichia pastoris special features will be addressed to demonstrate how its full molecular capacity can be exploited when employed for production processes at different levels of scale. During hands-on laboratory sessions and small-group workshops, the participants will clarify and discuss suitable, tailor-made solutions for selected case studies.

Participants will be provided with supporting materials to guide them through the course.

Based on practical experience gained during this course, participants will acquire structured guidance on how to achieve optimum production performance using Pichia.

Iskandar Dib (VTU Technology GmbH, Graz, Austria)

Katharina Ebner (bisy e.U., Hofstätten/Raab, Austria)

Jasmin Fischer (bisy e.U., Hofstätten/Raab, Austria)

Anton Glieder (TU Graz, Austria)

Michaela Gerstmann (TU Graz, Austria)

Anna Hatzl (TU Graz, Austria)

Karin Kovar (daspool, Wädenswil, Switzerland)

Knut Madden (BioGrammatics Inc., Carlsbad, California, U.S.A.)

Harald Pichler (TU Graz, Austria)

Hana Raschmanova (UCT Prague, Czech Republic)

Christian Schmid (TU Graz, Austria)

Marcel Straumann (daspool, Wädenswil, Switzerland)

Gerhard Thallinger (TU Graz, Austria)

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Spots Left

*The number of places is limited and will be allocated on a first-come-first-served basis. Please, register by simple e-mail to Michaela Gerstmann. In response, you will receive an invoice and your registration will be guaranteed and binding.

Contact for registration: michaela.gerstmann@tugraz.at

Please, contact us for multiple partnering and support opportunities within the hands-on-pichia course and to enhance the visibility of your company or its product’s.

In this hands-on-pichia course 2018, experts will explain and demonstrate the efficient use of the Pichia pastoris (Komagataella phaffii) expression and production system.

We will explore underlying concepts and provide insights into current laboratory practices, step-by-step protocols and novel innovative tools. In particular, a number of Pichia pastoris special features will be addressed to demonstrate how its full molecular capacity can be exploited when employed for production processes at different levels of scale. During hands-on laboratory sessions and small-group workshops, the participants will clarify and discuss suitable, tailor-made solutions for selected case studies.

Participants will be provided with supporting materials to guide them through the course.

Based on practical experience gained during this course, participants will acquire structured guidance on how to achieve optimum production performance using Pichia.

Iskandar Dib (VTU Technology GmbH, Graz, Austria)

Katharina Ebner (bisy e.U., Hofstätten/Raab, Austria)

Jasmin Fischer (bisy e.U., Hofstätten/Raab, Austria)

Anton Glieder (TU Graz, Austria)

Michaela Gerstmann (TU Graz, Austria)

Anna Hatzl (TU Graz, Austria)

Karin Kovar (daspool, Wädenswil, Switzerland)

Knut Madden (BioGrammatics Inc., Carlsbad, California, U.S.A.)

Harald Pichler (TU Graz, Austria)

Hana Raschmanova (UCT Prague, Czech Republic)

Christian Schmid (TU Graz, Austria)

Marcel Straumann (daspool, Wädenswil, Switzerland)

Gerhard Thallinger (TU Graz, Austria)

2,050 € for industry participants 

(plus optionally 300 € for a short pitch enhancing your company’s visibility)

1,450 € for participants from academia

1,250 € for students (with a valid university enrolment ID)

These course fees cover:

  • entry to all workshops, laboratory and technology review sessions
  • all course and laboratory materials
  • the welcome reception
  • meals and drinks during the course
  • social programme including two dinners
  • a voucher* to support further working with Pichia
    (*terms and conditions will be detailed at the course)

Please note that course fees do not include accommodation

On-site registration:

Sunday, 2 September 2018, 3:30 to 4.00 p.m.

Monday, 3 September 2018, 1:00 to 1.30 p.m.

Supporters 2018